The player can meet and duel Serenity after meeting both her brother Joey and Yami Yugi. Serenity comments that Joey's enthusiasm for the game got her hooked into it as well, and borrows some of his phrases. She appears as a regular opponent in the first map, and is also available as an opponent for the player before the Finals of the Beginner and Expert Cups.
One night after defeating both Serenity and Dox at least once, Dox will kidnap her to lure the player into a Shadow Game against him for revenge. The player must win to rescue Serenity.
Much later, Serenity expresses concern over Joey after he disappears during the confrontations against the Rare Hunters, and is grateful when he is freed from Yami Marik's control.
Curiously, despite Nightmare Troubadour mostly borrowing elements from the anime, Serenity's design in this game is closer to her depiction in the manga.